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Design of the smartphone-based FC device.(A) Photographs of the3D-printed device prototype. Video S1 isa short clip of the device in operation. (B) Renderings of the modelof the prototype device from different viewpoints. The surroundingbox is omitted for clarity. A schematic for the laser optics and adiagram and photograph of the microfluidic chip can be found in the SI.

Counting of SK-BR3 and MDA-MB-231 cells in parallel using SiO2@(QD540-CM-Dex)-(anti-HER2) and SiO2@(QD650-CM-Dex)-(anti-MUC1),respectively. (A) Cartoon schematic of the experiment (not drawn toscale). (B) Example of a frame from a smartphone FC video. Frame imagebrightness has been digitally increased for display purposes. An intensityprofile across selected cells of each type can be found in Figure S17. Video S4 is a 20 s clip from one of the smartphone FC videos. (C) Examplesof normalized G/B and R/B intensity ratios and cell classificationsfor five samples with an increasing number of MDA-MB-231 cells andan approximately constant number of SK-BR3 cells (see Figure S18 for additional data sets). Normalizationof intensity ratios assisted with application of the SVM trainingset to the experimental data (see the SI for details). (D) Correlation plots for the measured and expectednumbers of SK-BR3 and MDA-MB-231 cells, and for the measured and expectedratios of MDA-MB-231 and SK-BR3 cells. This plot includes the samplesin panel (C) and in Figure S18. 041b061a72